Standardized 3D in vitro Tumour-Stromal Model for Breast Cancer in biomimetical Hydrogels for the Testing of Tumour Therapeutic Agents
The goal of the project is the establishment of a standardized 3D in vitro tumour-stromal model for breast cancer for applications in medical-pharmaceutical research.
The basis of in vitro models are inert 3D-Life hydrogels. By building in components of the extra-cellular matrix (EZM), receptor-specific peptides, endothel cells and fibroblasts in the 3D-Life hydrogels, as well as by changing the mechanical hydrogel characteristics, a controllable tumour-stromal interaction model will be developed in which the influence of tumour therapeutic agents can be tested under realistic conditions. In contrast to current 3D cell-culture models, in the new model approach, all influencing parameters of the stroma can be altered and controlled.
The research project will also seek to establish processes of microscopic and molecular analysis of cell clusters and the newly formed EZM.
Cellendes GmbH, Reutlingen
PolyQuant GmbH, Bad Abbach
NMI an der Universität Tübingen, Reutlingen
Duration: 2013 – 2016, 36 months
The project is funded by the Federal Ministry of Education and research (BMBF) through the "FHprofUnt" funding stream of the "Research at Universities of Applied Sciences" project.